Cell - Gut Immune Maturation Depends on Colonization with a Host-Specific Microbiota

Cell - Gut Immune Maturation Depends on Colonization with a Host-Specific Microbiota

Gut Immune Maturation Depends on Colonization with a Host-Specific Microbiota

• Summary
• Introduction
• Results
• Discussion
• Exp. Proc.
• Data
• References
• Supp. Info.
• Related Info.
• Comments (0)

Loading...

• Graphical Abstract
• PaperClip

• Click to listen to the interview. You can browse the article while listening. Rollover in the blue bar above to access the audio player.

To listen to this audio file, you need to download the latest Adobe® Flash Player.
Note: You can download and play the audio file using the "Download this Audio" link below.

Download this Audio (2.67 MB)

• PDF 2.36 MB
• Extended PDF 5 KB
• Export Citation
• Permissions

Cell, Volume 149, Issue 7, 1578-1593, 22 June 2012
Copyright © 2012 Elsevier Inc. All rights reserved.
10.1016/j.cell.2012.04.037

Referred to by: Host and Microbes Date Exclusively

Authors

Hachung Chung, Sünje J. Pamp, Jonathan A. Hill, Neeraj K. Surana, Sanna M. Edelman, Erin B. Troy, Nicola C. Reading, Eduardo J. Villablanca, Sen Wang, Jorge R. Mora, Yoshinori Umesaki, Diane Mathis, Christophe Benoist, David A. Relman, Dennis L. KasperSee Affiliations

• Hint: Rollover Authors and Affiliations

Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA Departments of Microbiology and Immunology and of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA Department of Medicine, Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA Yakult Central Institute for Microbiological Research, Yaho 1796, Kunitachi, Tokyo 186-8650 begin_of_the_skype_highlighting 186-8650 end_of_the_skype_highlighting, Japan Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA Division of Infectious Diseases, Children's Hospital Boston, Boston, MA 02115, USA Corresponding author Present address: Tempero Pharmaceuticals, Cambridge, MA 02139, USA

• Highlights
► Mouse and human microbiota differ in bacterial species, primarily within Firmicutes ► Human microbiota (HMb) colonized mice have a global immunodeficiency like GF mice ► HMb induced less T cell proliferation and activation than mouse microbiota (MMb) ► HMb mice are more susceptible to enteric and disseminated infection than MMb mice

Summary

Gut microbial induction of host immune maturation exemplifies host-microbe mutualism. We colonized germ-free (GF) mice with mouse microbiota (MMb) or human microbiota (HMb) to determine whether small intestinal immune maturation depends on a coevolved host-specific microbiota. Gut bacterial numbers and phylum abundance were similar in MMb and HMb mice, but bacterial species differed, especially the Firmicutes. HMb mouse intestines had low levels of CD4⁺ and CD8⁺ T cells, few proliferating T cells, few dendritic cells, and low antimicrobial peptide expression—all characteristics of GF mice. Rat microbiota also failed to fully expand intestinal T cell numbers in mice. Colonizing GF or HMb mice with mouse-segmented filamentous bacteria (SFB) partially restored T cell numbers, suggesting that SFB and other MMb organisms are required for full immune maturation in mice. Importantly, MMb conferred better protection against Salmonella infection than HMb. A host-specific microbiota appears to be critical for a healthy immune system.