Infect Genet Evol. 2013 Nov 21;21C:214-219. doi: 10.1016/j.meegid.2013.11.004. [Epub ahead of print]
Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record.
Mijatovic-Rustempasic S1, Teel EN1, Kerin TK1, Hull JJ1, Roy S1, Weinberg GA2, Payne DC1, Parashar UD1, Gentsch JR1, Bowen MD3.
Abstract
In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8∗ region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.
Published by Elsevier B.V.
KEYWORDS:
Rotavirus, USA, VP4 protein, VP7 protein, VP8(∗), Vaccine
- PMID:
- 24270016
- [PubMed - as supplied by publisher]
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